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Microarray

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he Microarray Core Facility is a component of the UAlbany Center for Functional Genomics and was established to provide the infrastructure to enable the development of major projects that utilize DNA microarrays for large scale analysis of gene expression. The DNA Microarray Center currently has the ability to run microarrays from various vendors (Affymetrix GeneChip, Agilent Sureselect and Nimblegen) as well as others that have been developed by academia or other organizations on a standard slide format. We offer microarray services to probe gene expression, gene regulation, exon splicing as well as epigenetics.

The DNA Microarray Center also provides bioinformatics tools for further analysis of the results of microarray experiments.

The Facility provides the following services to investigators from any academic institution as well as corporate customers.

Affymetrix GeneChip Analysis
Affymetrix GeneChip

Affymetrix´s proprietary GeneChip technology utilizes photolithography based in–situ synthesis of arrays containing 25–mer oligonucleotide probes. We provide gene expression (3´–IVT, Gene ST and Exon ST), gene regulation (Tiling), miRNA, genotyping on the GeneChip platform. The core has a lot of experience in working with small samples (LCM, biopsies, flow sorted cells and immunoprecipitated material)

Agilent microarray Analysis
Agilent Microarray Agilent´s proprietary SurePrint technology uses an inkjet, non–contact printing process to produce glass arrays containing 60–mer oligonucleotide probes.We provide gene expression (custom or whole genome), gene regulation (Promoter Tiling), miRNA, epigenetic pr ofiling (DNA Methylation) on the Agilent platform. The core has a lot of experience in working with small samples (LCM, biopsies, flow sorted cells and immunoprecipitated material)
Nimblegen microarray Analysis
Nimblegen´s technology utilizes a maskless photolithography based in–situ synthesis of arrays containing up to 70–mer oligonucleotide probes. We currently provide gene expression and gene regulation (Promoter tiling) and epigenetic profiling (DNA methylation) services on the Nimblegen platform.
Superarray Analysis
The core can provide services on qPCR arrays provided by SABiosciences/Qiagen.
Custom microarray analysis
The core can also provide services on any custom arrays supplied by the end user. We have used many such slides spotted at other institutions for 1– or 2–color microarray analysis. Protein or cytokine arrays have also been scanned at our facility.
Data mining Services
The Core Facility has a variety of academic and commercial data mining software including GeneSpring (Agilent), Agilent Genome Workbench, Roche DEVA and Expression Console (Affymetrix).
Validation of expression analysis data
qPCR data The facility offers a myriad of services for validation of gene expression data including Sybyr Green and Taqman quantitative PCR assays.

For more information, we invite you to contact Dr Sridar Chittur at 518-591-7215 or at schittur@albany.edu.

 

Instrumentation | Fees for Services | Sample Preparation, Handling and QC | Procedure for Submitting Samples
Turn around Time | Frequently Asked Questions | Useful Links


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