The Microarray Core is well equipped for detailed studies on gene expression as well as related services including RNA isolation and quality control; hybridizations to Affymetrix GeneChip and in house spotted cDNA arrays. Major instrumentation at our core facility include the following:
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Agilent BioAnalyzer
A small amount of sample is loaded into the wells in a chip which is then loaded into the Bioanalyzer. Electrodes applied to the chip cause the RNA to move through microchannels filled with a sieving polymer and fluorescent dye. Fluorescence signal is plotted against run-time to generate an electropherogram or translated to gel-like images. Quality metrics such as 28S/18S ratio and RIN (RNA integrity number) are generated also.
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Affymetrix GeneChip Fluidics station 400
The fluidics stations automate the washing and staining aspects of the sample processing stream
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Affymetrix GeneChip Scanner
An excitation laser is passed over the surface of themicroarray at a very high resolution.
Emmissions are scanned at multiple wavelengths from a single excitation wavelength.
Autoloader allows temperature controlled handling of up to 48 GeneChips per run.
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Axon Genepix Scanner
An excitation laser is passed over the surface of themicroarray at a very high resolution.
Emmissions are scanned at multiple wavelengths from a single excitation wavelength.
Used for scanning spotted arrays on glass slides, one at a time.
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Nanodrop ND-1000
The Nanodrop ND-1000 is a "cuvette-less" spectrophotometer. One microliter of sample is
placed on the pedestal and the Nanodrop stretches the liquid into a column of fixed length.
Fiberoptics is employed to pass filtered light through the stretched column. Absorbance at various
wavelengths is measured and reported through an attached computer.
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GMS Microarrayer
A robotic system used for creating custom spotted arrays.
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GSI Lumonics 3-laser array scanner
A multipurpose 3-laser slide array scanner
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Biomek 2000 workstation
The Biomek workstation is a liquid handling system for general purpose plates setup
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