Molecular Biology

DNA Sequencing | Customized Services | Quantitative PCR

CFG Custom Molecular Biology Services

A wide range of custom services in molecular biology are available at the CFG. These include project-based services such as gene cloning and construction of insertion plasmids, gene discovery and characterization, gene expression studies, and recombinant protein expression and characterization.

Gene Cloning

• Restriction digestion and analysis
• PCR, RT-PCR
• Plasmid construction and purification
• Site-directed mutagenesis

Gene Discovery/Characterization

• cDNA library screening
• Isolation of cDNA by RT-PCR
• Identification of complete cDNA by RACE analysis
• Genomic library screening
• Isolation of genes by long range PCR cloning

Gene Expression

• Quantitative Real Time PCR
• RT-PCR
• Northern analysis
• Microarray analysis
• Western, immunoprecipitation analysis

Protein Expression/Characterization

• Generation of expression constructs
• Western, immunoprecipitation analysis

To arrange for custom services, please contact Dr. John Tine, Director of the Molecular Genetics Core Facility, by phone (518-591-7212) or e-mail.

DNA Sequencing | Customized Services | Quantitative PCR