Molecular Biology

DNA Sequencing | Customized Services | Quantitative PCR

CFG DNA Sequencing Services

Template Concentrations | Primer Concentrations | Policy on Repeat Reactions
Quality Control | Standard Primers | Template Preparation

Quality Control

We take significant steps to quality control each sequencing run in our facility. We include a sequencing standard (consisting of a standard plasmid sequenced with a standard primer) in each run. If we do not obtain good quality sequence from this standard, it is indication that there may have been a general problem with the reactions, and thus any failed reactions in such a run would be repeated at no charge to the investigator. Rarely, we find improper loading of a capillary or some other failure of a specific capillary. To compensate for this, every sequencing reaction is split in two and loaded in two separate capillaries. Thus, in the event of an isolated problem with a capillary we can obtain data from the other member of the sample pair. If both members of the pair fail, it is unlikely that a loading problem, etc, has occurred with both capillaries. We would then proceed with our resequencing policy as stated above.

DNA Sequencing | Customized Services | Quantitative PCR