| Service |
Unit |
Academic |
| Molecular Genetics |
| Oligo annealing |
Pair |
$20.00 |
| DNA Sequencing (template w/ 1 primer) |
Reaction |
$10.00 |
| Genomic DNA isolation, midi |
Sample |
$50.00 |
| Genomic DNA isolation (blood, clinical), mini |
Sample |
$55.00 |
| Genomic DNA isolation, genotyping, mini |
Sample |
$25.00 |
| Total RNA isolation, midi |
Sample |
$55.00 |
| mRNA isolation, midi |
Sample |
$120.00 |
| Total RNA isolation (blood), mini |
Sample |
$60.00 |
| PCR w/ gel analysis |
Reaction |
$10.00 |
| PCR w/ purification |
PCR product |
$45.00 |
| Library screen, genomic |
Screen |
$3,500.00 |
| Library screen, cDNA |
Screen |
$3,500.00 |
| Construction of transgenic/KO insertion vector |
Plasmid |
$3,250.00 |
| Taqman qPCR optimization |
Primer pair per probe |
$950.00 |
| Taqman qPCR, per sample |
Sample |
$20.00 |
| Taqman RT-qPCR, per sample |
Sample |
$30.00 |
| SybrGreen qPCR optimization |
Primer pair |
$1,250.00 |
| SybrGreen qPCR, per sample |
Sample |
$15.00 |
| SybrGreen RT-qPCR, per sample |
Sample |
$25.00 |
| qPCR primer/probe selection |
Primer pair/probe |
$20.00 |
| Bioinformatics |
| Bioinformatics |
Hour |
$60.00 |
| Proteomics/Mass Spectrometry |
| SDS-PAGE |
One protein |
$60.00 |
| 2D Gel Electrophoresis |
IPG strip |
$150.00 |
| SyProRuby stain |
Gel |
$20.00 |
| Imaging and analysis |
Gel |
$40.00 |
| Enzymatic Digest |
One protein |
$100.00 |
| HRMS |
Sample |
$85.00 |
| MW by LC/MS |
Sample |
$100.00 |
| MW by MALDI |
Sample |
$50.00 |
Protein ID by LC-MS/MS |
Sample |
$180.00 |
| Protein phosphopeptide ID |
Spot |
$680.00 |
| MudPIT protein profiling |
Step |
$100.00 |
| ITRAQ protein profiling |
Set |
4 plex $4,000 (> 25 fractions) 8 plex $5,000 (> 25 fractions) |
| De novo sequencing |
One peptide |
$75/hr |
| HPLC (Agilent) |
hour day |
$35 $85 |
| FPLC (Amersham) |
hour day |
$35 $85 |
| Microarray Services |
Prices do not include cost of GeneChips.
GeneChips can be purchased from CFG at discounted prices or directly from Affymetrix.
GeneChip Arrays should be provided by user at time of sample submission. |
| Premier service (RNA isolation to data acquisition) |
Sample |
$400.00 |
| Full service (cDNA synthesis to data acquisition) |
Sample |
$340.00 |
| Custom Services |
| RNA isolation from tissue |
Sample |
$60.00 |
| RNA BioAnalyzer QC (up to 12 samples) |
Sample |
$70.00 |
| Labeled cRNA synthesis from total RNA |
Sample |
$310.00 |
| Rehybridization only (previously labeled sample) |
Sample |
$75.00 |
| Data analysis only |
Hour |
$75.00 |
| Transgenic and Knockout Mice |
| Pronuclear injection |
Construct |
$3,000 |
| Embryo isolation |
Procedure |
$250 |
| Electroporation of ES cells |
Construct |
$7,500 |
| Blastocyst injections |
Construct |
$4,500 |
| Karyotyping |
Clone |
$180 |
| ES screening |
Construct |
$1,500 |
| ES expansion |
Clone |
$150 |
| Mycoplasma |
Clone |
$150 |
| Flow Cytometry |
| Flow Cytometry |
Hour |
$65.00 |
| Cell sorting, set-up |
Set-up |
$150.00 |
| Cell sorting |
Hour |
$75.00 |
| Cell Culture |
| Cell Line Passage, T25 flask |
Flask |
Call for pricing |
| Cell Line Passage, T75 flask |
Flask |
Call for pricing |
| Cell Line Passage, T150 flask |
Flask |
Call for pricing |
| Cell Line Passage, media change |
Change |
Call for pricing |
| Stable transfection |
Cell line |
Call for pricing |