CFG Staff

Qishan Lin

Director,
UAlbany Proteomics Facility
Adjunct Assistant Professor
Biomedical Sciences

Academic Background

• Ph.D. (Analytical Chemistry) 1996, Chinese Academy of Sciences, China
• MSc. (Organic Chemistry) 1991, Chinese Academy of Sciences, China
• B.S. (Chemistry) 1988, National University of Defense and Technology, China

Professional Background

• Chief Proteomics Scientist, Myomatrix Therapeutics, Rensselaer, NY (2001-2002)
• Postdoctoral Fellow, Albany Medical College, Albany, NY (2001-2002)
• Research associate, Howard Hughes Medical Institute, Seattle, WA (1997-2000)
• Postdoctoral Fellow, Department of Biochemistry, University of Washington, Seattle, WA (1997-2000)
• Research Associate Professor, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China (1997)
• Practice Research Assistant Professor, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China (1991-1996)

Research Experience

• Comparative study of phycobiliprotein from four types of marine blue and red alga (Bangia fusco-purpurea, Porphyra haianensis, P.yezoensis and Spirulina platensis) with respect to their separation techniques, structure and functions
• Study on synthesis of new type of chromatographic media and its application in bonded phase of reversed, hydrophobic, ion exchange, size exclusion and affinity chromatography
• Purification and characterization of transthyretin from human blood, and Development of new labeling reagent for sub-fmol level mono-saccharide analysis for glyco-protein by HPLC and HPCE
• Enzymatic study of a phosphatidic acid-preferring phospholipase A1 (PA-PLA1) from bovine testis using vesicle assay system; Identification and sequencing species- or tissue-specific iso-forms of PA-PLA1 in bovine sperm cells by means of high-resolution two dimensional gel electrophoresis and Nano-HPLC/electrospray mass spectrometry
• Using liposome artificial membrane to probe the kinetics of the interfacial enzymes
• Structural and functional study of a novel angiotensinase from a failing human heart; Proteomic approach to profile membrane proteins in a failing human heart vs. non-failing human heart; Proteomic charaterization neonatal rat cardiomyocytes; Application of proteomics approach to identify trans-regulatory networks of myosin heavy chain
• Application proteomics approaches including high-resolution 2D electrophoresis, multidimensional chromatography, mass spectrometry, and database to infer and describe biological process

Research Interests

The current interest focuses on the methodology development for proteomics. While genomics based on DNA sequencing is now reaching a mature state, an understanding of gene expression and subsequent protein translation has lagged behind. Recent advances in mass spectroscopy related technologies promise to bring protein biochemistry to a level that permits high throughput, global, analysis of protein expression. The application of this technology to biological systems has been termed "proteomics." Proteomics serves as a platform for global analysis of protein, and aims at analyzing biologically relevant proteins in health and disease state. The current research interest is to develop new technologies for the comprehensive analysis of regulated biological systems and apply these technologies to study the signal transduction pathways that induce and control the hypertrophy of cardiac myocytes under different load conditions. The approach is based on the integration of high-resolution protein separation techniques including two dimensional electrophoresis, high performance liquid chromatography as well as capillary electrophoresis with mass spectrometers. As the number of the gene sequences determined is rapidly increasing, we anticipate that technologies that focus on the characterization of proteins will become essential tools for the comprehensive analysis of biological systems.

Selected Publications

• Snelling, W., Lin,Q., Moore, J., et al. A proteomic analysis of Cryptosporidium parvum and of protein expression during excystation, Mol Cell Proteomics (2006), In press

• Manning, M., Hesham, H., Lin, Q., Colon, W, The subproteome of kinetically stable proteins in Escherichia Coli, Mol Cell Proteomics (2006), Accepted.

• Decaprio, A.P., Lopachin, R.M., and Lin, Q., Neurofilament protein adduct formation in spinal core fractions of 2,5 -hexanedione intoxicated rats (2006)

• Cismasiu, V.B., Adamo, K., Gecewicz, J., Duque, J., Lin, Q., and Avram, D. BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter (2005), Oncogene, 24: 6753-64

• Lin, Q., Keller RS, Weaver B., and Zisman LS. Interaction of ACE2 and integrin b1 in failing human heart (2004), Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1689(3), 175-178

• Zisman LS, Keller RS, Weaver B., Lin, Q., Speth R., Bristow MR., Canver CC, Increased Angiotensin-(1-7)-Forming Activity in Failing Human Heart Ventricles. Evidence for Upregulation of the Angiotensin-Converting Enzyme Homologue ACE2 (2003), Circulation, 108, 1707-1712

• Lin, Q., Higgs HN, and Glomset, JA, Membrane lipids have multiple effects on interfacial catalysis by a phosphatidic acid-preferring phospholipase A1 from bovine testis (2000), Biochemistry, 39 (31), 9335-9344

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