CFG Staff

John Tine

Director, Molecular Genetics Core Facility
Adjunct Assistant Professor

Academic Background

• Ph.D. (Microbiology and Immunology) 1989, Albany Medical College, Albany, NY
• B.S. (Biology) 1982, Siena College, Loudonville, NY

Professional Background

• Team Leader/Senior Scientist, Virogenetics Corporation, Troy, NY (1998-2000)
• Malaria Vaccine Project Leader, Aventis Pasteur, Marcy L'Etoile, France (1998-2000)
• Senior Scientist, Virogenetics Corporation, Troy, NY (1997-1998)
• Research Scientist, Virogenetics Corporation, Troy, NY (1995-1996)
• Scientist, Virogenetics Corporation, Troy, NY (1992-1995)
• Post-doctoral Fellow,Virogenetics Corporation, Troy, NY (1989-1992)

Honors and Awards

• 3 granted US/International patents, 16 US/International patents pending.
• Senior Research Scholar 1985-1989, Albany Medical College, Albany, NY
• Trustee Scholar 1982-1984, Albany Medical College, Albany, NY

Research Experience

• Expression and characterization of genes encoding antigens from infectious agents and tumor-associated antigens in poxviruses.
• Processing and presentation by dendritic cells of T cell epitopes from tumor associated antigens
• Development of vaccine candidates for human malaria based on recombinant viral and DNA plasmid vectors expressing malarial antigens
• Development of candidate immunotherapies for melanoma and colorectal cancer based on recombinant viral and DNA plasmid vectors expressing tumor-associated antigens.
• Molecular biological assays for quality control of recombinant poxvirus vaccine clinical lots
• Design of clinical trials to assess safety, immunogenicity, and efficacy of poxvirus-based vaccine and immunotherapy candidates
• Molecular genetic characterization of the evolution of non-classical MHC genes in the mouse.

Selected Publications

• Rogers, W.O., Baird, J.K., Kumar, A., Tine, J.A., Weiss, W.R., Aguiar, J.C., Gowda, K., Gwadz, R.W., Kumar, S., Gold, M., and Hoffman, S.L. 2001.
  Multi-Stage, Multi-Antigen, Heterologous Prime Boost Vaccine for Plasmodium knowlesi Malaria Provides Partial Protection in Rhesus Macaques.
  Infection and Immunity, in press.

• Schultz, E.S., Zhang, Y., Knowles, R., Tine, J., Traversari, C., Boon, T., and van der Bruggen, P. 2001.
  A MAGE-3 peptide recognized on HLA-B35 and HLA-A1 by cytolytic T lymphocytes.
  Tissue Antigens 57, 103-109.

• Horig, H., Lee, D.S., Conkright, W., Divito, J., Hasson, H., LaMare, M., Rivera, A., Park, D., Tine, J., Guito, K., Tsang, K.W., Schlom, J., and Kaufman, H.L. 2000.
  Phase I Clinical Trial of a Recombinant Canarypoxvirus (ALVAC) Vaccine Expressing Human Carcinoembryonic Antigen and the B7.1 Co-Stimulatory Molecule.
  Cancer Immunology and Immunotherapy 49, 504-514.

• Sutmiller, R.P.M., Schurmans, L.R.H.M., Tine, J.A., van der Voort, E.I.H., Melief, C.J.M., Jager, M.J., and Offringa, R. 2000.
  Adoptive T Cell Immunotherapy of Human Uveal Melanoma Targeting gp100.
  Journal of Immunology 165, 7308-7315.

• Luiten, R.M., Demotte, N., Tine, J., and van der Bruggen, P. 2000.
  A MAGE-A1 Peptide Presented to Cytolytic T Lymphocytes by Both HLA-B35 and HLA-A1 Molecules.
  Tissue Antigens 56, 77-81.

• Sedegah, M., Weiss, W., Sacci Jr., J.B., Charoenvit, Y., Hedstrom, R., Gowda, K., Fallarme, V., Tine, J., Kumar, S., Hobart, P., and Hoffman, S.L. 2000.
  Improving Protective Immunity Induced by DNA-Based Immunization: Priming with Antigen and GM-CSF Encoding Plasmid DNA and Boosting with Antigen Expressing Recombinant Poxvirus.
  Journal of Immunology 164, 5905-5912.

• Wang, R., Doolan, D.L., Charoenvit, Y., Hedstrom, R.C., Gardner, M.J., Hobart, P., Tine, J., Sedegah, M., Fallarme, V., Sacci, J.B., Kaur, M., Klinman, D.M., Hoffman, S.L., and Weiss, W.R. 1998.
  Simultaneous Induction of Multiple Antigen-Specific CTL in Non-Human Primates by Immunization with a Mixture of Four Plasmodium falciparum DNA Plasmids.
  Infection and Immunity 66, 4193-4202.

• Sedegah, M., Jones, T.R., Kaur, M., Hedstrom, R., Hobart, P., Tine, J.A., and Hoffman, S.L. 1998.
  Boosting with Recombinant Vaccinia Increases Immunogenicity and Protective Efficacy of a Malaria DNA Vaccine.
  Proceedings of the National Academy of Sciences USA 95, 7648-7653.

• Ockenhouse, C.F., Sun, P.F., Lanar, D.E., Wellde, B.T., Hall, B.T., Kester, K., Stoute, J., Magill, A., Kryzch, U., Farley, L., Wirtz, R.A., Sadoff, J.C., Kaslow, D.C., Kumar, S., Church, L.W.P., Crutcher, J.M., Wizel, B., Hoffman, S., Lalvani, A., Hill, A.V.S., Tine, J.A., Guito, K.P., de Taisne, C., Anders, R., Horii, T., Paoletti, E., and Ballou, W.R. 1998.
  Phase I/IIa Safety, Immunogenicity, and Efficacy of NYVAC-Pf7, a Pox-Vectored, Multiantigen, Multistage Vaccine Candidate for Plasmodium falciparum Malaria.
  Journal of Infectious Diseases 177, 1664-1673.

• Tine, J.A., Lanar, D.E., Smith, D.M., Wellde, B.T., Schultheiss, P., Ware, L.A., Kauffman, E.B., Wirtz, R.A., de Taisne, C., Hui, G.S.N., Chang, S.P., Church, P., Kaslow, D.C., Hoffman, S., Guito, K.P., Ballou, W.R., Sadoff, J.C., and Paoletti, E. 1996.
  NYVAC-Pf7: A Poxvirus-Vectored, Multiantigen, Multistage Vaccine Candidate for Plasmodium falciparum Malaria.
  Infection and Immunity 64, 3833-3844.

• Lanar, D.E., Tine, J.A., de Taisne, C., Seguin, M.C., Cox, W.I., Winslow, J.P., Ware, L.A., Kauffman, E.B., Gordon, D., Ballou, W.R., Paoletti, E., and Sadoff, J.C. 1996.
  Attenuated Vaccinia Virus-Circumsporozoite Protein Recombinants Confer Protection Against Rodent Malaria.
  Infection and Immunity 64, 1666-1671.

• Tine, J.A., de Taisne, C., Conseil, V., Delplace, P., Camus, D., and Paoletti, E. 1992.
  Immunogenicity of the Plasmodium falciparum Serine Repeat Antigen (p126) Expressed by Vaccinia Virus.
  Infection and Immunity 61, 3933-3941.

• Tine, J.A., Walsh, A., Rathbun, D., Leonard, L., Wakeland, E.K., Dilwith, R., and Flaherty, L. 1990.
  Genetic Polymorphisms of Q Region Genes From Wild-derived Mice: Implications for Q Region Evolution.
  Immunogenetics 31, 315-325.

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